Steady-state fast inactivation changes in a similar way for Nav1.7 and L858F. A. Voltage dependences of steady-state fast inactivation for Nav1.7 at 16°C (filled squares, n = 12), 25°C (filled triangles, n = 11) and 35°C (filled circles, n = 9). Availability was assessed using a 500 ms prepulse ranging from -150 mV to 0 mV followed by a 40 ms test pulse to -20 mV. The current was normalized to the largest current response evoked by the test pulse. Steady-state inactivation curves were fitted with a Boltzmann equation, as described in methods. B. Voltage dependences of steady-state fast inactivation for L858F at 16°C (open squares, n = 9), 25°C (open triangles, n = 11) and 35°C (open circles, n = 10). C. Temperature dependence of the midpoint of steady-state inactivation for Nav1.7 (black bars) and L858F (white bars) at the indicated temperatures. D. The slope factor of steady-state inactivation for Nav1.7 (black bars) and L858F (white bars) plotted versus temperature. * indicate significant differences between values with p < 0.05, tested with ANOVA and Tukey HSD post hoc analysis.