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Figure 1 | Molecular Pain

Figure 1

From: Phosphorylation of ERK in neurokinin 1 receptor-expressing neurons in laminae III and IV of the rat spinal dorsal horn following noxious stimulation

Figure 1

Phosphorylation of ERK in the ipsilateral dorsal horn after different types of noxious stimulus. pERK-immunostaining in confocal images of parasagittal sections through the medial part of the left dorsal horn from rats that were perfused with fixative 5 mins after the end of different types of noxious stimulus: a repeated pinching of the skin of the foot for 1 min (pinch), b immersion of the hindpaw in water at 52°C for 1 min (heat), c injection of 100 μl 2% formaldehyde into the hindpaw (form). These sections were also immunostained for PKCγ (not shown) and the two dashed lines, which outline lamina IIi, were drawn from the location of the plexus of PKCγ-immunoreactive dendrites (for further details see text). The positions of laminae I, IIo and III are also indicated in a. In all cases there is strong pERK-immunoreactivity that is mainly restricted to laminae I and IIo. Some pERK-immunoreactive cells were seen ventral to the IIo/IIi border, particularly after pinch. All images are projections of z-series consisting of 10 optical sections at 2 μm spacing. Scale bar = 100 μm.

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Molecular Pain

ISSN: 1744-8069

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