Figure 10

An inactive structural U0126 analogue (U0124) and a selective PKC inhibitor (GF109203X) have no effect on pain behavior. (A-C) Application of U0124 (100 μM, 15 min) into the CeLC had no effect on the significantly increased audible and ultrasonic vocalizations and on the decreased hindlimb withdrawal thresholds of arthritic rats (n = 3). (D-F) GF109203X (100 μM, 15 min) administered into the CeLC had no effect on the significantly increased vocalizations (n = 9) and on decreased withdrawal reflexes (n = 5) of arthritic rats. Vocalizations and withdrawal reflexes were measured as in Figure 8 (see Methods for details). Bar histograms and error bars represent mean ± SE. * P < 0.05, ** P < 0.01, *** P < 0.001 (repeated-measures ANOVA with Bonferroni post-tests).