Figure 3

RTX blocks inflammatory heat hyperalgesia when applied directly to the sciatic nerve. Both percutaneous and open field injection of RTX (250 ng, 50 μl) inhibited inflammatory heat hyperalgesia following carrageenan injection (6 mg, 150 μl). There was no significant difference in latency time regarding RTX application method (i.e. percutaneously vs. direct open field application); therefore data were pooled (N = 15). However, for this set of animals, there was a significant increase in normal heat latency as compared to vehicle treated animals (*P < 0.05, 1 way ANOVA, Scheffe post-hoc analysis). Animals pretreated with vehicle (0.25% Tween-80, PBS 50 μl, N = 10) had a significant decrease in their heat withdrawal latency (⁺P < 0.05, 1 way RM-ANOVA) following induction of inflammation.