Figure 6

Expression of Na channels Na _v 1.1 through Na _v 1.9 in cultured DRG cells before and after GRO/KC incubation. cDNA template was reverse-transcribed from RNA that had been isolated from control cells and cells from the sister cultures treated overnight with GRO/KC (1.5 nM). Expression of each gene was normalized to that of the housekeeping gene HPRT in the same batch of cDNA, determined during the same PCR amplification. A, profile of Na channel expression relative to HPRT. B, fold-change in expression of the 3 highly expressed Na channels after GRO/KC treatment. *, significantly different from control (= 1.0, dotted line), ratio t-test. Values shown are averages from 4 separate cultures.