Figure 6

Endogenously produced DAG activates TRPV1 channels in a heterologous expression system. (a) Activation of muscarinic receptor M3 by 30 μM Acetylcholine (Ach) opened TRPV1 channels (a, upper left panel) in HEK 293 cells coexpressing M3- and TRPV1 (upper left panel). Patch pipette solution contained both 1 μM chelerythrine and 10 μM RHC80267 Ach-induced currents were almost completely blocked by 10 μM CZP (upper right panel). Ach-induced currents were not observed in HEK 293 cells expressing only M3 (lower left panel). (b) Summary of Ach-evoked currents. Asterisks represent a significant difference by ANOVA test followed by Tukey – Kramer multiple comparison test (n. s: non-significant, ** p < 0.001).