Figure 7
From: Direct activation of Transient Receptor Potential Vanilloid 1(TRPV1) by Diacylglycerol (DAG)
OAG binds to the capsaicin binding site. (a) An illustration of the topology of TRPV1. The capsaicin binding site and putative PKC and CaMKII phosphorylation sites were shown. (b) Like capsaicin, OAG failed to activate inward currents in Y511A and S502A/T704I mutants of TRPV1. In contrast, OAG-evoked currents were produced in S502A/S800A and Δ774–838 mutants of TRPV1. (c) Summary of OAG (O) and capsaicin (C) evoked current responses in the TRPV1 mutants indicated in b. (d) Currents evoked by 100 nM capsaicin were partially blocked by 100 μM OAG. The remaining currents between the two arrows were considered to be OAG-induced currents. (e) Summary of OAG (O) and CAP (C) evoked currents. The asterisk showed significant difference in normalized OAG-induced current (paired t-test, p < 0.05).