Figure 5
From: Cutaneous tactile allodynia associated with microvascular dysfunction in muscle

Lipid peroxidation, cytokines and NFêB induced by IR injury, and effects of inhibitiors on allodynia. a, Colorimetric assay shows there is significantly greater MDA, mmol/μg) in the ipsilateral (ipsi, n = 7), compared to contralateral (contra, n = 7) CPIP or sham (n = 6) HPDM muscle at 2 hrs post-reperfusion (*P < 0.05). b, c, Reduced pre-drug CPIP PWTs (g) (compared to baseline (Bas)) are significantly and dose-dependently elevated by systemic treatment with NAC (n = 7,6,6,6 for vehicle (Veh), 10,50,200 mg/kg) (b) or TEMPOL (n = 7,6,6,6 for vehicle, 40,100,250 mg/kg) (c) in day 2 CPIP rats (*P < 0.05 compared to pre-drug). d, e, f, ELISA shows there is significantly greater TNFα (pg/ml, n = 9,9,8 for 5 min, 2 h, 48 h) (*P < 0.05)(d), IL-6 (pg/ml, n = 9,9,9 for 5 min, 2 h, 48 h) (*P < 0.05) (e), and IL-1β (pg/ml, n = 9,9,9 for 5 min, 2 h, 48 h) (*P < 0.05) (f) in the HPDM of CPIP, as opposed to sham-treated rats (n = 9,9,8 (d), n = 9,9,9 (e), n = 9,9,9 (f) for 5 min, 2 h, 48 h) at various times post-reperfusion. g, ELISA shows there is significantly greater NFκB p50 (ng/ml protein) in CPIP compared to sham HPDM at 2 hrs (n = 18,15 for CPIP, sham) and 2 days post-reperfusion (n = 18,15 for CPIP, sham) (*P < 0.05), but not 7 days (n = 6,14 for CPIP, sham). h,i, Reduced pre-drug CPIP PWTs (g) (compared to baseline (Bas)) are significantly elevated by intraplantar treatment with IL-1RA (n = 9,4,9,9 for vehicle, 25,50,100 μg) (*P < 0.05 compared to pre-drug) (h) or systemic PDTC (n = 7,9,9,7 for vehicle, 10,30,100 mg/kg) (*P < 0.05 compared to pre-drug) (i) on day 2 post-reperfusion. All data expressed as mean ± s.e.m.