Figure 5

Lipid peroxidation, cytokines and NFêB induced by IR injury, and effects of inhibitiors on allodynia. a, Colorimetric assay shows there is significantly greater MDA, mmol/μg) in the ipsilateral (ipsi, n = 7), compared to contralateral (contra, n = 7) CPIP or sham (n = 6) HPDM muscle at 2 hrs post-reperfusion (*P < 0.05). b, c, Reduced pre-drug CPIP PWTs (g) (compared to baseline (Bas)) are significantly and dose-dependently elevated by systemic treatment with NAC (n = 7,6,6,6 for vehicle (Veh), 10,50,200 mg/kg) (b) or TEMPOL (n = 7,6,6,6 for vehicle, 40,100,250 mg/kg) (c) in day 2 CPIP rats (*P < 0.05 compared to pre-drug). d, e, f, ELISA shows there is significantly greater TNFα (pg/ml, n = 9,9,8 for 5 min, 2 h, 48 h) (*P < 0.05)(d), IL-6 (pg/ml, n = 9,9,9 for 5 min, 2 h, 48 h) (*P < 0.05) (e), and IL-1β (pg/ml, n = 9,9,9 for 5 min, 2 h, 48 h) (*P < 0.05) (f) in the HPDM of CPIP, as opposed to sham-treated rats (n = 9,9,8 (d), n = 9,9,9 (e), n = 9,9,9 (f) for 5 min, 2 h, 48 h) at various times post-reperfusion. g, ELISA shows there is significantly greater NFκB p50 (ng/ml protein) in CPIP compared to sham HPDM at 2 hrs (n = 18,15 for CPIP, sham) and 2 days post-reperfusion (n = 18,15 for CPIP, sham) (*P < 0.05), but not 7 days (n = 6,14 for CPIP, sham). h,i, Reduced pre-drug CPIP PWTs (g) (compared to baseline (Bas)) are significantly elevated by intraplantar treatment with IL-1RA (n = 9,4,9,9 for vehicle, 25,50,100 μg) (*P < 0.05 compared to pre-drug) (h) or systemic PDTC (n = 7,9,9,7 for vehicle, 10,30,100 mg/kg) (*P < 0.05 compared to pre-drug) (i) on day 2 post-reperfusion. All data expressed as mean ± s.e.m.