Hot plate response latency nociceptive behavior measurements. Hot plate response latency measurements are shown for naïve animals and animal groups with alcohol and high-fat induced pancreatitis. Hot plate test was conducted at baseline before induction of pancreatitis and for ten weeks subsequently. Note the significant shortening of hot plate response latencies for rats on the high fat and alcohol diet after week 3, indicating sensitization. The HSV-ENK treatment (arrow) significantly abrogated the shift in response latency for at least four weeks. Four weeks is typical of HSV vector expression.