Figure 1
E 2 -induced inhibition of I Gly in cultured SDH and HIP neurons. (A) Representative traces of current induced by 100 μM glycine in the presence or absence of E2 at various concentrations in cultured HIP (upper) and SDH (bottom) neurons. The neurons were pre-treated with E2 for 30 s before E2 and glycine were co-applied. (B) Summarized data illustrating the concentration dependence of E2 inhibition (n = 4–8) as shown in A. (C) E2 significantly inhibited I Gly recorded from the outside-out patches (n = 5). The upper traces show the representative I Gly recorded from outside-out patches in the presence and absence of E2. ***P < 0.001, Paired Student's t-test, compared with control without adding E2. (D) The concentration-response curves of I Gly in the presence and absence of 10 μM E2. For each neuron recorded, the current was normalized to the peak amplitude of I Gly induced by 100 μM glycine alone (✰) from the same neuron and each point represents the average value of 5–9 neurons.