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Figure 5 | Molecular Pain

Figure 5

From: Activation of cytosolic phospholipase A2 in dorsal root ganglion neurons by Ca2+/calmodulin-dependent protein kinase II after peripheral nerve injury

Figure 5

cPLA 2 and CaMKII are activated in the plasma membrane after applying ATP. (A) Increase in the levels of p-cPLA2 and p-CaMKII immunofluorescence and translocation of these proteins to the plasma membrane in neurons 5 min after the application of 10 μM ATP compared with controls. Similar results were observed in each of three experiments. Scale bar, 20 μm. (B) The association between p-cPLA2 and p-CaMKII after application of 10 μM ATP. Protein samples were subjected to immunoprecipitation with the anti-p-cPLA2 antibody, and isolated complex was separated by electrophoresis and immunoblotted with anti-p-cPLA2 and anti-p-CaMKII antibodies.

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Molecular Pain

ISSN: 1744-8069

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