Figure 5
From: Intrathecal delivery of PDGF produces tactile allodynia through its receptors in spinal microglia
Immunofluorescence of phosphorylated PDGF β-receptors and expression of PDGF receptor mRNAs in rats after nerve injury. (A) The immunoreactivity for p-PDGFRβ was detected in the L5 spinal dorsal horn 4 days after nerve injury. Scale bar, 200 μm. (B) The intensity of p-PDGFRβ immunofluorescence was quantified in the dorsal horn region of contralateral side (contra) and ipsilateral side (ipsi) of nerve injured rats. Data represent the means ± SEM of the immunofluorescence intensity (n = 3). **P < 0.01 vs contra by Student's t-test. (C) Double immunofluorescence labeling of p-PDGFRβ (green) with OX-42 (red), a microglia marker. Scale bars, 20 μm. (D) Total RNA extracted from the L5 spinal cords of naive rats and peripheral nerve injured rats was subjected to quantitative analysis of PDGFR mRNA expression by real-time RT-PCR. Data are means ± SEM of the percentage over the naive value (ipsilateral side/contralateral side, n = 5).