Figure 4

Capsaicin or resiniferatoxin increases ROS production in mouse primary DRG cultures. (A) Photomicrographs of ROS in mouse DRG in primary cultures at baseline. (B) ROS production increases after resiniferatoxin stimulation. The effects are indicated by fluorescent dye intensity changes captured with epifluorescent microscopy and quantified with MetaMorphe image analysis. (C) Capsaicin increases ROS production at 30 minutes (**P < 0.01 compared to control) and ROS scavenger PBN (2 mM) blocks capsaicin induced ROS production at 30 minutes (^# P < 0.05 compared to capsaicin). (D) Resiniferatoxin increases ROS production at 30 minutes (***P < 0.001 compared to control) and PBN (2 mM) blocks resiniferatoxin induced ROS production at 30 minutes (^### P < 0.001 compared to resiniferatoxin). (E) PBN also significantly blocks ROS production at two lower doses (0.4 mM (***P < 0.001 compared to resiniferatoxin) and 0.08 mM (**P < 0.01 compared to resiniferatoxin)).