Number of TDAG8-expressing neurons increases in DRG after CFA-induced inflammation. After CFA-injection, lumbar 4–5 DRG ipsilateral and contralateral to injected paws were sectioned and hybridized with dig-labeled anti-sense mTDAG8 probes, followed by co-staining with antibodies against peripherin (PERI, green fluorescence) and N52 (red fluorescence). (A) Phase-contrast fields (a, d) are neurons labeled with cRNA probes. Fluorescence images (b, e) show neurons labeled with green (PERI only), red (N52 only), and yellow (PERI and N52). Phase-contrast images and fluorescence images were combined to obtain merged images (c, f). Arrows indicate the peripherin-positive neurons labeled with antisense probes. Arrowheads are the N52-positive neurons labeled with antisense probes. The scale bar is 50 μm. (B) The histogram shows the percentage of total neurons that expressed TDAG8 in PERI, N52, or overlapping subpopulations. (C) The histogram shows the percentage of total TDAG8-positive neurons that co-localized with PERI or N52 markers.