Table 1 Primer sequences employed for RT-PCR analysis
Gene | Primer sequences (5' to 3') | Product length (bp) |
|---|---|---|
P2X1 (forward) | TGATCTGGACTGGCACGTTC | 441 |
P2X1 (reverse) | GGTCCTCATGTTCTCCTGCA | Â |
P2X3 (forward) | TGGCG TTCTG GGTAT TAAGA TCGG | 743 |
P2X3 (reverse) | CAGTG GCCTG GTCAC TGGCG A | Â |
P2X4 (forward) | GAGGC ATCAT GGGTA TCCAG ATCAA G | 447 |
P2X4 (reverse) | GAGCG GGGTG GAAAT GTAAC TTTAG | Â |
P2X6 (forward) | CGATT CACTC TCCAG TCCG | 317 |
P2X6 (reverse) | GGTCC TCCAG TAGAA ACCG | Â |
P2Y2 (forward) | CGCTT CAACG AGGAC TTCA | 589 |
P2Y2 (reverse) | CCATG AGCAC GTAAC AGAC | Â |
P2Y4 (forward) | GTTGC CTATG AGCTA TGCAG | 518 |
P2Y4 (reverse) | ACCAT GACTG CCGAA CTGAA | Â |
P2Y6 (forward) | GGAGA CCTTG CCTGC CGCCT GGTA | 410 |
P2Y6 (reverse) | TACCA CGACA GCCAT ACGGG CCGC | Â |
β-actin (forward) | TTCTA CAATG AGCTG CGTGT GGC | 456 |
β-actin (reverse) | CTC(A/G)T AGCTC TTCTC CAGGG AGGA | 982 |
G3PDH (forward) | TGAAGGTCGGTGTGAACGGATTTGGC | Â |
G3PDH (reverse) | CATGTAGGCCATGAGGTCCACCAC | Â |