Figure 3
Sensitivity of P2X2/3 currents to changes in PIP 2 and PIP 3 levels in Xenopus oocytes. A) Sample traces showing the effect of 35 μM wortmannin incubation and the injection of PBS, PIP2 or PIP3 as compared to an initial current recorded prior to incubation. B) Pooled data of P2X2/3 responses to 2 h incubation in control solution (Barth's solution with DMSO), in 35 μM wortmannin, 100 nM wortmannin, or 35 μM LY294002 (N = 10–33). C) Pooled data of P2X2/3 current inhibition by 2 h incubation with 35 μM wortmannin in oocytes injected with vehicle, PIP2, and PIP3 (N = 5–17). (**, P < 0.01; ***, P < 0.001)