Skip to main content
Figure 3 | Molecular Pain

Figure 3

From: Signal Transduction Mechanisms Underlying Group I mGluR-mediated Increase in Frequency and Amplitude of Spontaneous EPSCs in the Spinal Trigeminal Subnucleus Oralis of the Rat

Figure 3

Effects of D-AP5, U73122 and 2-APB on the DHPG-induced increase of sEPSC frequency and amplitude. Representative sEPSC traces recorded from Vo neurons were sampled before and during bath application of 10 μM DHPG (5 min) in the presence of the NMDA receptor antagonist D-AP5 (50 μM; Aa), the PLC inhibitor U73122 (10 μM; Ba) and the IP3 receptor inhibitor 2-APB (100 μM; Ca). Time-course graphs demonstrate the DHPG-induced changes of mean frequency (Ab, Bb and Cb) and amplitude (Ac, Bc and Cb) in D-AP5 (n = 5), U73122 (n = 6) and 2-APB (n = 6). Numbers on the graphs indicate the corresponding time of the traces sampled. Histograms compare magnitudes of DHPG-induced increases in sEPSC frequency (D) and amplitude (E) during the application (filled) or the washout (open) of DHPG in the presence of D-AP5 (n = 5), U73122 (n = 6) and 2-APB (n = 6). Asterisks indicate significant differences of DHPG effects, compared to the effect in the Krebs condition (**P < 0.01, *P < 0.05).

Back to article page

Molecular Pain

ISSN: 1744-8069

Contact us