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Figure 1 | Molecular Pain

Figure 1

From: Recombinant adeno-associated virus serotype 6 (rAAV2/6)-mediated gene transfer to nociceptive neurons through different routes of delivery

Figure 1

rAAV2/6 transduction of the dorsal root ganglia following subcutaneous, intramuscular, sciatic nerve and intrathecal delivery. (A) Macroscopic view of L4 DRG of mice injected with rAAV2/6 expressing eGFP. From left to right: uninjected, dose response of sciatic nerve injected, subcutaneous (high dose), intramuscular (high dose), intrathecal (high dose). (B) Native eGFP expression and corresponding bright field image in a typical 12 μm L4 DRG section following sciatic nerve delivery. Scale bar, 100 μm. (C) Transduction rates within L4 DRG following subcutaneous, intramuscular, sciatic nerve delivery at three doses (n = 3 per group and dose) and intrathecal delivery at the highest dose (n = 5) expressed as a percentage of eGFP expressing profiles per total number of DRG cells observed. LD, low dose (2.6 × 104 tu); MD, medium dose (8.0 × 104 tu); HD, high dose (2.6 × 105 tu). (D) qPCR against the vector genome (beta globin intron of rAAV2/6) in DRG, spinal cord and liver for the high dose of the four administration routes. (E) Reverse transcription qPCR against eGFP mRNA transcripts normalized with GAPDH mRNA in the DRG for the various protocols.

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Molecular Pain

ISSN: 1744-8069

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