Table 1 Primary antibodies used in immunohistochemistry of CANs.
Label | Host | Dilution | Company |
|---|---|---|---|
NR1 | Mouse | 1:500 | BD Biosciences |
NR2B | Mouse | 1:500 | BD Biosciences |
NR2D | Rabbit | 1:100 | Santa Cruz |
NK1r | Mouse | 1:500 | Zymed |
SP | Rabbit | 1:250 | Chemicon |
PAR-2 | Chicken | 1:500 | Aves Lab, Inc. |
TRPV1 | Rabbit | 1:500 | Affinity Bioreagents |
CGRP | Rabbit | 1:250 | Courtesy of Dr. Michael Iadarola |
nNOS | Rabbit | 1:250 | Chemicon |
VIP | Rabbit | 1:500 | Chemicon |
NF 200 | Mouse | 1:250 | Sigma |
IB4 | Goat conjugated to IB4 | 1:1000 | Invitrogen |
N1 | Rabbit | 1:500 | Courtesy of Dr. Michael Iadarola |
C1 | Rabbit | 1:250 | Courtesy of Dr. Michael Iadarola |
Alexa Fluor 488 | Goat anti-Rabbit | 1:1000 | Invitrogen |
Alexa Fluor 488 | Donkey anti-Mouse | 1:1000 | Invitrogen |
Alexa Fluor 488 | Goat anti-chicken | 1:1000 | Invitrogen |