Effect of methanandamide on GlyR-mediated synaptic transmission in SDH and DDH neurons. A plot showing GlyR-mediated mIPSC frequency (holding potential - 70 mV) in an SDH neuron during bath application of methAEA (5 μm, upper bar). mIPSC frequency declines in the presence of methAEA. Middle traces are averaged mIPSCs (n = 15) under control conditions, and after 10 minutes in methAEA. Black bars above x-axis indicate when averaged mIPSCs were obtained. Plot on right presents group data summarising proportional changes in GlyR-mediated mIPSC properties in methAEA. mIPSC frequency was reduced in methAEA. mIPSC peak amplitude, rise time, and decay time-constant were unaltered. B Effect of methAEA on GlyR-mediated mIPSCs in DDH neurons (data presented in same format as A). methAEA also reduced GlyR-mediated mIPSC frequency in DDH neurons without altering mIPSC peak amplitude, rise time, or decay time-constant. Note, each data point in left panels in A and B represent averaged instantaneous mIPSC frequency, binned in 15 s intervals.