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Figure 2 | Molecular Pain

Figure 2

From: Effective relief of neuropathic pain by adeno-associated virus-mediated expression of a small hairpin RNA against GTP cyclohydrolase 1

Figure 2

rAAV constructs and its gene silencing effect. (a) 293T cells were co-transfected with or without shGCH1 (pshGCH1) in the presence of pcDNA3-rGCH1 for two days. (b) Schematic illustration of rAAVs encoding GFP and shGCH1; GFP and shGCH1 expression cassettes were located in opposite directions to minimize interference with each other. (c) HeLa cells were co-treated with pcDNA3-rGCH1 and rAAV at the designated multiplicity of infection (MOI) for two days. Western-blotting analyses revealed a significant decrease in the rGCH1 levels in the presence of shGCH1. MT; mock-treated. ITR; internal terminal repeat of rAAV.

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Molecular Pain

ISSN: 1744-8069

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