Immunohistochemical analysis of the L5 spinal cord section. (A-C) Immunofluorescent micrographs of CK1ε (red) in L5 spinal cord ipsilateral to sham- (A), SNL- (B) operated C57BL/6J mice, and SNL-operated Ca
2.2-/- mice (C) co-labeled with NeuN (Ai-Ci), GFAP (Aii-Cii) or Iba 1(Aiii-Ciii) shown in green. Scale bar, 100 μm. (D, E) Mean number of GFAP-positive spinal astrocyte (D) and Iba 1-positive microglia (E). Mouse antibody against CK1ε was used in Aiii-Ciii and rabbit antibody against CK1ε was used in Ai-Ci and Aii-Cii. There was always a tendency that the intensity with the mouse monoclonal antibody is generally stronger and the background is relatively higher than with the rabbit polyclonal antibody, although the specificity of staining was found to be similar between the two antibodies. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA followed by Tukey's post hoc test); n = 3-4.