Figure 3
From: Facilitation of synaptic transmission and pain responses by CGRP in the amygdala of normal rats
Inhibition of PKA, but not PKC, blocks synaptic effects of CGRP. (A) Original recordings of monosynaptic EPSCs (average of 10 EPSCs) evoked at the PB-CeLC synapse. Facilitatory effects of CGRP (100 nM) were blocked by co-administration of a PKA inhibitor (KT5720, 1 μM). (B) Summary of time course data for the sample of CeLC neurons (n = 7) show the inhibitory effects of KT5720 were reversible after washout. Peak amplitudes of EPSCs recorded during drug application were expressed as percent of predrug control values (set to 100%). (C) Individual traces (average of 8-10) of monosynaptic EPSCs show that the facilitatory effects of CGRP (100 nM) were not blocked by co-administration of a PKC inhibitor (GF109203x, 1 μM). (D) Summary of time course data for the sample of CeLC neurons show that the effects of CGRP did not desensitize during drug application for 30 min (n = 6; display as in (B)). Symbols and error bars represent mean ± SEM. * P < 0.05; n.s. (not significant), P > 0.05 (paired t-test, comparing the last measurement before and during application of cAMP-RP or KT5720). Statistical analysis was performed on raw data.