NMDA receptor antagonist blocks CGRP effects. (A) Original recordings of monosynaptic EPSCs (average of 8-10 EPSCs) evoked at the PB-CeLC synapse in the presence of AP5 (50 μM). CGRP (100 nM) had no effect. (B) Normalized data for the sample of CeLC neurons (n = 4). Peak amplitudes of EPSCs recorded during drug application were expressed as percent of predrug control values (set to 100%). (C) Individual traces (average of 8-10) of monosynaptic EPSCs show that the facilitatory effects of CGRP (100 nM) were blocked by co-administration of AP5 (50 μM). (D) Normalized data for the sample of CeLC neurons (n = 4; display as in (B)). Bar histograms show mean ± SEM. n.s. (not significant), P > 0.05 (paired t-test), ** P < 0.01 (ANOVA with Bonferroni posttests). Statistical analysis was performed on raw data.