Effect of chemokines on the motility of microglia in spinal dorsal horn. (A-G). Local application of 10 ng/ml monocyte chemoattractant protein-1 (MCP-1), fractalkine (FKN), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), interleukin 1β (IL-1β), interleukin 6 (IL-6) or tumor necrosis factor α (TNF-α) had no effect on microglia motility. The merged picture is the overlay of imaging at 0 min (green) and 30 min (red) after drug application. Figs. (E-G) showed the results recorded on amoeboid cells. Bars equal to 20 μm.