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Table 2 Primer and TaqMan probe sequences for the rat genes characterized in this experiment

From: Down-regulation of Toll-like receptor 4 gene expression by short interfering RNA attenuates bone cancer pain in a rat model

Gene Primer forward Primer reverse
β-actin 5'-CACCCGCGAGTACAACCTTC-3' 5'-CCCATACCCACCATCACA CC-3'
Probe* 5'-FAM-TCCTACCCCCAATGTGTCCGTCGTG-TAMRA-3'
TLR4 5'-AGCTTTGGTCAGTTGGCTCT-3' 5'-CAGGATGACACCATTGAAGC-3'
Probe* 5'-FAM-AACAGCAACCTCTAAAGCTCATGGCA-TAMRA-3'
IL-6 5'-AAGGACCAAGACCATCCAAC-3' 5'-ACCACAGTGAGGAATGTCCA-3'
Probe* 5'-FAM-TCGGCAAACCTAGTGTGCTATGCC-TAMRA-3'
IL-1β 5'-GCAACTGTCCCTGAACTCAA-3' 5'-TGTCAGCCTCAAAGAACAGG-3'
Probe* 5'-FAM-TCATTCTCCTCACTGTCGAAAGCTGC-TAMRA-3'
TNF-a 5'-CTAACTCCCAGAAAAGCAAGCAA-3' 5'-CCTCGGGCCAGTGTATGAGA-3'
Probe* 5'-FAM-CAGCCAGGCAGGTTCCGTCC-TAMRA-3'
INF-β 5'-CAGCTGAATGGAAGGCTCAAC-3' 5'-CGGGTGCATCACCTCCATA-3'
Probe* 5'-FAM-CAGCTACAGGACGGACTTCAAGATC-TAMRA-3'
CD11b 5'-GCCTCCAAGTCCGCAAGAA-3' 5'-TCATAAGTGACAGTGCTCTGGATGT-3'
Probe* 5'-FAM-ACCAAGGACAGGCTGCGAGA AGGA-TAMRA-3'
CD14 5'-CGGGAACTGACTCTTGAAAACC-3' 5'-CATCCAGAAGCGGCGAAA-3'
Probe* 5'-FAM-CGAGGTAACCGGCACCGCG-TAMRA-3'
  1. *The TaqMan probe has a reporter fluorescent dye, 6-carboxyfluorescein (FAM), at the 5' end, and a fluorescence dye quencher, 6-carboxytetramethylrhodamine (TAMRA), at the 3' end.
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Molecular Pain

ISSN: 1744-8069

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