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Table 2 Primer and TaqMan probe sequences for the rat genes characterized in this experiment

From: Down-regulation of Toll-like receptor 4 gene expression by short interfering RNA attenuates bone cancer pain in a rat model

Gene

Primer forward

Primer reverse

β-actin

5'-CACCCGCGAGTACAACCTTC-3'

5'-CCCATACCCACCATCACA CC-3'

Probe*

5'-FAM-TCCTACCCCCAATGTGTCCGTCGTG-TAMRA-3'

TLR4

5'-AGCTTTGGTCAGTTGGCTCT-3'

5'-CAGGATGACACCATTGAAGC-3'

Probe*

5'-FAM-AACAGCAACCTCTAAAGCTCATGGCA-TAMRA-3'

IL-6

5'-AAGGACCAAGACCATCCAAC-3'

5'-ACCACAGTGAGGAATGTCCA-3'

Probe*

5'-FAM-TCGGCAAACCTAGTGTGCTATGCC-TAMRA-3'

IL-1β

5'-GCAACTGTCCCTGAACTCAA-3'

5'-TGTCAGCCTCAAAGAACAGG-3'

Probe*

5'-FAM-TCATTCTCCTCACTGTCGAAAGCTGC-TAMRA-3'

TNF-a

5'-CTAACTCCCAGAAAAGCAAGCAA-3'

5'-CCTCGGGCCAGTGTATGAGA-3'

Probe*

5'-FAM-CAGCCAGGCAGGTTCCGTCC-TAMRA-3'

INF-β

5'-CAGCTGAATGGAAGGCTCAAC-3'

5'-CGGGTGCATCACCTCCATA-3'

Probe*

5'-FAM-CAGCTACAGGACGGACTTCAAGATC-TAMRA-3'

CD11b

5'-GCCTCCAAGTCCGCAAGAA-3'

5'-TCATAAGTGACAGTGCTCTGGATGT-3'

Probe*

5'-FAM-ACCAAGGACAGGCTGCGAGA AGGA-TAMRA-3'

CD14

5'-CGGGAACTGACTCTTGAAAACC-3'

5'-CATCCAGAAGCGGCGAAA-3'

Probe*

5'-FAM-CGAGGTAACCGGCACCGCG-TAMRA-3'

  1. *The TaqMan probe has a reporter fluorescent dye, 6-carboxyfluorescein (FAM), at the 5' end, and a fluorescence dye quencher, 6-carboxytetramethylrhodamine (TAMRA), at the 3' end.