Skip to main content

Table 2 ADP evokes Ca++ transients through P2Y1.

From: Gi- and Gq-coupled ADP (P2Y) receptors act in opposition to modulate nociceptive signaling and inflammatory pain behavior

 

10 μM ADP

100 μM ADP

MRS2179

100 μM ADP

wildtype

   

% responders

35 ± 5%

52 ± 6%

8 ± 3%

n (cells)

37

197

40

wildtype

   

response size (ΔF)

0.09 ± 0.01

0.53 ± 0.05

0.18 ± 0.02

wildtype

   

response area (ΔFarea)

1 ± 2

11 ± 2

6 ± 1

P2Y1-/-

   

% responders

ND

9 ± 4%

10 ± 1%

n (cells)

 

100

55

P2Y1-/-

   

response size (ΔF)

ND

0.90 ± 0.28

1.31 ± 0.32

P2Y1-/-

   

response area (ΔFarea)

ND

15 ± 3

18 ± 4

  1. Dramatically fewer sensory P2Y1-/- neurons produce ADP-evoked calcium transients compared to wildtype neurons. Remaining responses in P2Y1-/- neurons are similar in size to those seen in wildtype. Similar effects were obtained using MRS2179, a P2Y1-specific antagonist. Frequency and response magnitude values are mean ± SEM.
Back to article page

Molecular Pain

ISSN: 1744-8069

Contact us