Figure 6

Distribution of PAP-II in DRG neurons 2 days after nerve injury. (A) Double-immunofluorescence staining shows that the majority of PAP-II-positive small DRG neurons are IB4-, CGRP- and NPY-negative 2 days after sciatic nerve axotomy. However, ~60% of the PAP-II-containing DRG neurons are peripherin-immunoreactive. Double arrowheads, single arrowhead and arrow indicate PAP-II-positive neurons co-expressing other molecules, PAP-II-negative neurons but containing other molecules, and PAP-II-positive neurons absent for other molecules, respectively. Scale bar = 50 μm. (B) Quantitative analysis shows that 10.9 ± 1.6% PAP-II-immunoreactive DRG neurons (n = 271) also express CGRP 2 days after nerve axotomy, while 12.6 ± 2.0% (n = 217) and 8.9% ± 1.7% (n = 379) PAP-II-positive DRG neurons are stained for IB4 or NPY respectively. More than half of the PAP-II-containing DRG neurons (54.6 ± 10.3%, n = 254) are peripherin-immunoreactive neurons.