Figure 2

Effect of NR2C/D preferring antagonist on NMDA induced current recorded from EGFP+ neurons. A, NMDA induced inward current in an EGFP+ dorsal horn neuron. Current responses to voltage ramps during NMDA application are cut off at the peak of the response to allow direct comparison of the NMDA response to that in B. Current - voltage relationship obtained at the peak of the NMDA response is plotted below with the g(-90 mV) and g(MIC) indicated by dotted lines and VMIC indicated by dashed lines. B, Current responses to voltage ramps during co-application of 15 μM NMDA and 25 μM UBP141, an NR2C/D preferring antagonist, to the same cell as in A. Current - voltage relationship from the peak response is again plotted below with the g(-90 mV) and g(MIC) indicated by dotted lines and VMIC indicated by dashed lines. C shows both individual and average g(-90 mV)/g(MIC) values in the absence and presence of UBP141 (*P < 0.05). D indicates individual and average VMIC values in the absence and presence of UBP141 (*P < 0.05). For C and D, perforated lines indicate the conductance ratio of NR2A/B or NR2C/D receptor induced currents and VMIC derived from oocyte expression data of Kuner and Schoepfer [19].