Figure 3

Effect of NR2A/B preferring antagonist on NMDA induced current recorded from EGFP+ neurons. A, NMDA induced inward current in an EGFP+ dorsal horn neuron. Current responses to voltage ramps during NMDA application are cut off at the peak of the response to allow direct comparison of the NMDA response to that in B. Current - voltage relationship obtained at the peak of the NMDA response is plotted below with the g(-90 mV) and g(MIC) indicated by dotted lines and VMIC indicated by dashed lines. B, current responses to voltage ramps during co-application of 15 μM NMDA and 100 nM EAB318, an NR2A/B selective antagonist, to the same cell as in A with the current -voltage relationship shown below. C, both individual and average g(-90 mV)/g(MIC) values in the absence and presence of EAB318 (*P < 0.05) are shown. D shows individual and average VMIC values in the absence and presence of EAB318 (*P < 0.05). For C and D, perforated lines indicate the conductance ratio of NR2A/B or NR2C/D receptor induced currents and VMIC derived from oocyte expression data of Kuner and Schoepfer [19].