Figure 6

Quantitative image analysis of GFP-expressing DRG neurons. A) A similar number of neurons smaller than 22 μm (≤22 μm), or larger than 22 μm (>22 μm) expressed GFP after both rAAV5-GFP and rAAV8-GFP injection. Both vectors preferentially targeted large neurons. B) Injection of rAAV5-GFP resulted in more GFP expression in CGRP-positive neurons smaller than 22 μm than was seen with injection of rAAV8-GFP; t-test, t_.05,2,4 = 3.421, p = 0.0268. Injection of either vector did not result in GFP expression in a substantial portion of the IB4-binding population. C) Approximately 80% of the DRG neurons expressing GFP were larger than 22 μm, regardless of whether rAAV5-GFP or rAAV8-GFP was injected. Approximately 20% of these GFP-expressing neurons were also CGRP positive.