Figure 4

Monosynaptic afferent fiber-evoked EPSCs in SG neurons of control and cancer-bearing mice. Monosynaptic Aδ and C fiber-evoked EPSCs in response to the stimulation of dorsal root attached to the spinal cord slices (A). The monosynaptic nature was clarified based on their constant latencies and absence of failures with repetitive stimulations (20 Hz and 2 Hz for Aδ and C fiber's responses, respectively; see Methods). Representative averaged traces of monosynaptic Aδ fiber-evoked EPSCs in control (left) and cancer-bearing (right) mice (B). There was no significant difference in the amplitude of monosynaptic Aδ fiber-evoked EPSCs between control (n = 13) and cancer-bearing (n = 14) mice (right) (C). Representative averaged traces of C fiber-evoked EPSCs in control (left) and cancer-bearing (right) mice (D). The amplitude of monosynaptic C fiber-evoked EPSCs was significantly increased in cancer-bearing (n = 12) than control mice (n = 10), P < 0.05 (Mann-Whitney U-test) (E). Values represent means ± S.E.M.