Survival of WT and KI trigeminal neurons in culture and their expression of Ca
2.1 protein. A, Survival is calculated as number of β-tubulin III positive cells per unit area after 1-4 days in culture. Data are normalized with respect to those at 1 day. n = 4, p > 0.05. B, Somatic size distribution of trigeminal neurons (β-tubulin III immunoreactive) in culture from WT and KI mice. n = 4. C, Immunocytochemical expression of CaV2.1 channels in intact trigeminal ganglia of WT and KI mice. Histograms represent% of CaV2.1 immunoreactive neurons over β-tubulinIII immunoreactive neurons in WT or KI ganglia. n = 3, p > 0.05. D, Example of western blot of protein extracts from WT and KI trigeminal ganglia or culture, probed with anti-CaV2.1 antibody. Equal loading was ensured by membrane probing with β-tubulinIII antibodies. n = 3, p > 0.05. Histograms (right) show no significant difference between these conditions.