Figure 5

The GRP-induced sIPSC facilitation is blocked by RC3095 and picrotoxin. (A and B) The effect of GRP (300 nM) could be blocked by RC3095 (3 μM, n = 4), suggesting that the GRP's action is mediated by GRPR (upper). Representative sIPSCs recorded in a pyramidal cell from a control mouse at a holding potential of +10 mV under baseline conditions (upper), during GRP application (middle), and after the GRPR antagonist was added (lower). (C) Time course of reversible drug effect of RC3095 (3 μM) to the enhancement of sIPSC induced by GRP treatment. (D) The effect of GRP (300 nM) could be blocked by picrotoxin (100 μM, n = 4). Representative sIPSCs recorded in a pyramidal neuron under baseline conditions (upper), during GRP application (middle), and after picrotoxin was added (lower). (E) Time course of reversible drug effect of picrotoxin (100 μM) to the enhancement of sIPSC induced by GRP treatment.