Figure 4
From: KATP channel subunits in rat dorsal root ganglia: alterations by painful axotomy
Colocalization studies in DRG neurons. A-C. Colocalization of BODIPY-Glybenclamide staining of SUR1 subunits (A), with anti-Kir6.2 antibody (B), showing that SUR1 subunits are co-expressed with Kir6.2 subunits in the same complexes (C: merged). D-F. Co-localization of anti-SUR1 antibody (D) with anti-CGRP antibody (E) in DRG studied under fluorescent microscopy. Merged image (F) shows anti-SUR1 immunofluorescence present in small, CGRP positive neurons, as well as in CGRP negative neurons. G-I. Co-localization of anti-SUR1 antibody (G) with anti-CGRP antibody (H) in dissociated neurons studied under confocal microscopy. Merged image (I) shows SUR1 immunofluorescence present in small, CGRP + neurons (arrows), as well as in CGRP negative neurons. K-M. Co-localization of anti-SUR1 antibody (K) with anti-Caspr antibody (L) in DRG studied under confocal microscopy. Merged image (M) shows that SUR1 immunofluorescence co-localizes with anti-Caspr staining in paranodal sites. Yellow arrowheads point to SUR1 positive SLI (in K). N-P. Colocalization of anti-Kir6.2 antibody (N) with anti-Caspr antibody (O) in DRG studied under confocal microscopy. Merged image (P) shows that anti-SUR1 immunofluorescence colocalizes with anti-Caspr staining in paranodal sites, adjacent to Ranvier's nodes (White arrows point at paranodal KATP channels). This colocalization of Caspr with SUR1 or Kir6.2 indicates that KATP channels of the Kir6.2/SUR1 subtype are present in paranodal sites (white arrows) adjacent to nodes of Ranvier. All samples are from slices within DRG tissue.