Table 2 PAR activators and concentrations used
From: Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones
PAR activator | Concentration | Selective for | Source |
|---|---|---|---|
Thrombin | 0.01-100 nM | PAR1,3,4 | Sigma, cat T4648 |
Cathepsin G | 1-1000 nM | PAR4 | Sigma, cat C4428 |
Trypsin | 0.1-10 μM | PAR1,2,3,4 | Sigma, cat T9201 |
Collagenase | 0.25% | - | Sigma, cat 4188 |
type IV | Â | Â | Â |
TFLLR-NH2 | 100 μM | PAR1 | Tocris, cat 1464 |
SFLLRN-OH (for rat) | 100 μM | PAR1 | Bachem, cat H8365 |
SLIGRL-NH2 | 100 μM | PAR2 | Tocris, cat 1468 |
AYPGKF-NH2 | 200 μM | PAR4 | Sigma, cat A3227 |