Table 3 Summary of characteristics of DRG neurons from adult and neonatal mice and rats
From: Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones
| Â | Adult mouse | Neonatal mouse | Adult rat | Neonatal rat |
|---|---|---|---|---|
% of THR-reponsive neurons (Ca imaging) | 17.5 | 15.2 | 14.5 | 19.2 |
% of THR-reponsive neurons (PKCε translocation) | 19,4 ± 1,2 | 17,5 ± 1,5 | 13,4 ± 2,4 | 17,8 ± 1,0 |
% of THR-responsive neurons which are IB4+ | 0,9 ± 0,1% | 1,0 ± 0,5% | 0 | 0 |
% of THR-responsive neurons responsive to PAR2-AP (Ca imaging) | 0 | 0 | 0 | 0 |
THR sensitizes TRPV1 (Ca imaging) | yes | yes | yes | yes |
PAR1-AP and PAR4-AP sensitize TRPV1 (patch clamp) | yes | - | - | yes |
Segregation between PAR1 and PAR2 responsiveness (Ca imaging) | yes | yes | yes | yes |
Neurons responsive to thrombin from day 1 in vitro | yes | yes | yes | yes |
Upregulation by NTN of thrombin-responsiveness (PKCε translocation) | yes | yes | yes | yes |
% of neurones responding to PAR2-AP expressing TRPV1 (Ca imaging) | 77,8 ± 9,4 | 91,0 ± 4,9 | 87,7 ± 6,2 | 88.6 ± 5.1% |
% of neurones responding to PAR2-AP which are IB4+ (Ca imaging) | 81.1 ± 8.3 | 81.8 ± 5,0 | - | 82.5 ± 6.0% |
% of THR-responsive non-neuronal cells in DRG cultures | 57,5 ± 5,6% | 62,5 ± 9,9% | 37,9 ± 7,0% | 55,4 ± 3,4% |