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Table 3 Summary of characteristics of DRG neurons from adult and neonatal mice and rats

From: Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones

  Adult mouse Neonatal mouse Adult rat Neonatal rat
% of THR-reponsive neurons (Ca imaging) 17.5 15.2 14.5 19.2
% of THR-reponsive neurons (PKCε translocation) 19,4 ± 1,2 17,5 ± 1,5 13,4 ± 2,4 17,8 ± 1,0
% of THR-responsive neurons which are IB4+ 0,9 ± 0,1% 1,0 ± 0,5% 0 0
% of THR-responsive neurons responsive to PAR2-AP (Ca imaging) 0 0 0 0
THR sensitizes TRPV1 (Ca imaging) yes yes yes yes
PAR1-AP and PAR4-AP sensitize TRPV1 (patch clamp) yes - - yes
Segregation between PAR1 and PAR2 responsiveness (Ca imaging) yes yes yes yes
Neurons responsive to thrombin from day 1 in vitro yes yes yes yes
Upregulation by NTN of thrombin-responsiveness (PKCε translocation) yes yes yes yes
% of neurones responding to PAR2-AP expressing TRPV1 (Ca imaging) 77,8 ± 9,4 91,0 ± 4,9 87,7 ± 6,2 88.6 ± 5.1%
% of neurones responding to PAR2-AP which are IB4+ (Ca imaging) 81.1 ± 8.3 81.8 ± 5,0 - 82.5 ± 6.0%
% of THR-responsive non-neuronal cells in DRG cultures 57,5 ± 5,6% 62,5 ± 9,9% 37,9 ± 7,0% 55,4 ± 3,4%