Figure 2

Percentage of galanin positive DRG neurons in double transgenic lines after axotomy. A, Representative DRG sections immunohistochemically stained for galanin one week after sciatic nerve axotomy from double transgenic and WT control. Animals were given water (including 5% w/v sucrose) (top panel) or dox (including 5% w/v sucrose) from the time of axotomy until sacrifice (lower panel). B, The percentage of galanin positive neurons in sections from axotomised L4 and L5 DRG with dox at 200 mg/ml (hatched bar) and without dox (solid bar). Double transgenic DRG have a significant overexpression of galanin after axotomy when compared to WT control animals in the absence of dox. No significant differences between transgenic and WT mice were observed when dox was administered (1-way ANOVA *** denotes P < 0.001).