Minocycline blocked the paclitaxel-induced mechanical allodynia. Application of paclitaxel (cumulative dose 24 mg/kg; 3 × 8 mg/kg) induced a marked and prolonged mechanical allodynia. On day 4, 8 and 12 after first paclitaxel treatment, the paclitaxel rats showed a significant decrease in 50% withdrawal threshold relative to the vehicle group. *P < 0.05; **P < 0.01. Minocycline pretreatment at dose of 30 mg/kg or 50 mg/kg significantly attenuated paclitaxel-induced mechanical allodynia compared with the corresponding time points of paclitaxel group, respectively. # P < 0.05; ##P < 0.01 and † P < 0.05; †† P < 0.01. However, 50% withdrawal threshold had no significant difference between the 30 mg/kg minocycline/paclitaxel group and the 50 mg/kg minocycline/paclitaxel group or the vehicle group. Minocycline at 30 mg/kg alone had no effect on the mechanical withdrawal threshold in control animals (n = 8/group).