Minocycline inhibited paclitaxel-induced loss of IENF. PGP9.5-staining shows epidermal innervation in the hind plantar paw skin of normal rats (A). Continuous loss of PGP9.5 positive nerve fibers is evident on day 2-12 following paclitaxel treatment (B-E), *P < 0.05; **P < 0.01 compared with the vehicle group. Minocycline pretreatment attenuated the loss of IENF (F, G), ##P < 0.01 compared with paclitaxel group at corresponding time points. Histogram represents the mean number of IENF per sight under various treated conditions (H) (n = 5/group).