Figure 5
From: Nociceptor-expressed ephrin-B2 regulates inflammatory and neuropathic pain
c-Fos expression was attenuated in the spinal cord in ephrin-B2 conditional mutant mice after formalin injection in the hindpaw. (A) Representative immunostaining for c-Fos in superficial laminae I, II after formalin injection. The right hemicord is showing the cord ipsilateral to the injection of formalin or saline. (B) Cell counting for c-Fos positive nuclei in the dorsal horn. Compared to Efnb2fl/fl control mice, the number of positive c-Fos nuclei in the ipsilateral side in Efnb2 CKO mutants is significantly reduced after formalin injection. Scale bar = 100 μm. All data presented as means ± SEM; using students t-test, * p < 0.05.