Figure 6

Increase of immunoreactivity for Iba1 was attenuated in the spinal cord in ephrin-B2 conditional mutant mice in a Seltzer neuropathic pain model. (A) Representative immunostaining for Iba1 in superficial laminae I, II after sciatic nerve partial ligation (Seltzer model). The right hemicord is showing the cord ipsilateral to the sciatic nerve partial ligation. (B) Cell counting for Iba1 positive cells in the dorsal horn. The microglia were activated after sciatic nerve partial ligation in both Efnb2^fl/fl controls and Efnb2 CKO mice. However, the increase of immunoreactivity for Iba1 was attenuated in Efnb2 CKO mice. Data shown as mean ± SEM, Scale bar = 100 μm. Using students t-test, * p < 0.05; ** p < 0.01.