Figure 1
From: Blocking spinal CCR2 with AZ889 reversed hyperalgesia in a model of neuropathic pain
mCCL2-induced intracellular calcium mobilization in HEK293 s cells expressing CCR2 is blocked by compound AZ889. (A) Mouse derivative of CCL2 evoked calcium activation of HEK293 s cells expressing CCR2 with an EC50 value of 1 nM. Calcium mobilization was recorded on a FLIPR system. (B) AZ889 dose-dependently inhibited CCR2-mediated calcium response evoked by 1 nM of mCCL2 with an IC50 value of 1.3 ± 0.2 nM (n = 5). The values were normalized to control mCCL2-evoked responses. The data shown is the average of 3 independent experiments.