Figure 2

Electrophysiological characteristics of CTx-FVIA and -MVIIA. (A) Representative traces of N-type Ca²⁺ currents (control) in C2D7 cells showing blockade by 100 nM CTx-FVIA. (B) Concentration-response curves for CTx-FVIA and -MVIIA (n = 3). (C) Time-course of the block induced by 100 nM CTx-FVIA or -MVIIV and its washout. Cells were held at -80 mV and depolarized to 0 mV. 100 nM CTx-FVIA (Δ) or CTx-MVIIA (○) were applied until a complete block was achieved and then washed out. Currents were measured every 15 s. (D) Potency of CTx-FVIA and -MVIIA and percent recovery after washout. (E) Averaged time constants τ_on for CTx-FVIA and -MVIIA determined from time-course graphs fitted with a three-parameter single exponential function.