Figure 4

EGF induces STAT3 translocation but not Erk1/2 phosphorylation. A) Kinetic of phosphorylation of Erk1/2 in response to 1 nM EGF treatment of DRG-neurons (n = 1000-2000 cells for each time point, error bars SEM). B) Dose response curve after 5 min or 10 min stimulation (n = 1000-3000 cells for each concentration, error bars SEM). C) 1 nM EGF induced phosphorylation and translocation of STAT3 into the nucleus of DRG-neurons. D) Quantification of neurons showing phosphorylated STAT3 in the nucleus in response to 1 nM EGF (p < 0.01; n = 300 cells per condition were counted in 3 independent experiments). E) Mean intensity of pSTAT3 increased in nuclei of neurons in response to 1 nM EGF treatment (p < 0.01; Mean intensity of nuclei (61 (control) and 83 (EGF treated)) were quantified in 3 independent experiments).