Generation of tamoxifen inducible Advillin Cre mouse. A - Diagram of the wt Advillin allele and the targeting construct. CreERT2 is inserted downstream of the initiation codon of Advillin. The construct also contained a Kanamycin selection gene that was excised from the BAC by Flp-recombination. B - Primer validation for screening of transgenic animals. AF - Advillin forward primer, AR - Advillin reverse primer, CF - Cre forward primer, CR - Cre reverse primer. The expected sizes of the PCR fragments are indicated. C - Southern blot with tail genomic DNA digested with HindIII and hybridized with the 5'homology arm probe. The bands for wildtype gene (wt) and transgene (tg) are indicated.