4-OHT induces Cre-expression in cell culture of neurons from dorsal root ganglia of AvCreERT2 mice. A - X-Gal, anti-peripherin (green) and anti-NF200 (red) staining of 4-OHT treated and uninduced cell cultures from DRG. Arrows indicate X-gal stained neurons. Note the punctuate nature of X-gal staining. B - Quantification of recombination events in untreated and 4-OHT treated cultures of neurons from DRGs. Data are presented as mean ± SEM. Statistical analysis - unpaired T-test, p ≤ 0.01. Scale bars = 40 μm.