Figure 6

Nitrosocysteine immunoreactivity in the spinal cord of inflammatory pain models. A. Immunoreactivity of nitrosocysteine in the spinal cord. Lumbar transverse sections (20 μm) of spinal cords prepared from before and 5 min, 6 h, and 24 h after injection of formalin, carrageenan, and CFA, respectively, were fixed and stained with anti-nitrosocysteine antibody as described in "Method". Bar = 100 μm. B. Quantification of S-nitrosocysteine immunostaining. Fluorescence intensity of the spinal cord was quantified by ImageJ. The intensity of the spinal cord in inflammatory pain models was normalized to that of naive mice, and the intensity (mean ± SEM, n = 23-24) of naive mice was taken as "1." **P < 0.01.