Figure 3

Exaggerated α,β-meATP-evoked response after neuropathic injury is blunted by PKC inhibition. (A) Sample traces of current responses to 10 μM α,β-meATP in presence of PKC inhibitors staurosporine (500 nM) and calphostin C (100 nM) in neuropathic DRG neurons under voltage-clamp settings. (B) Current density of peak responses to 10 μM α,β-meATP in presence of PKC inhibitors (N = 4-17, ** p < 0.005 *** p < 0.0005, compared to the neuropathic group). (C) Representative traces of 10 μM α,β-meATP-evoked P2X3 responses in naïve DRG neurons (left), and after pre-incubation with 500 nM staurosporine (right). (D) Current density of 10 μM α,β-meATP-evoked P2X responses with and without 500 nM staurosporine (N = 5, non significant difference).