Figure 9

GFL-induced enhancement in the stimulated-release of iCGRP is mediated by the Src kinase pathway. A) This representative Western blot demonstrates reduction in levels of c-Src and no change in Fyn levels in DRG in response to Src siRNA treatment. B and C) Densitometric analysis of three separate Western blots like that in A probing for B) c-Src and C) Fyn. D) Peptide release elicited by a 10 minute exposure to Hepes buffer alone (open bar) or Hepes buffer containing 50 nM capsaicin (Cap; dark bars) is expressed as mean percent total peptide content of cells in each well ± SEM (n = 12-18 wells per condition). Asterisks (*) indicate statistically significant differences in band density and iCGRP release between treatment groups and the no GFL condition using an ANOVA with Dunnett's post-hoc test (p < 0.05). Ampersands (@) indicate statistically significant differences between the GFL treatment and the siRNA treated condition using a t-test (p < 0.05). In all cases, release stimulated by capsaicin was significantly higher than basal release.